Comparison of Sodium Hypochlorite Digestion and Low-Temperature Ashing Preparation Techniques for Lung Tissue Analysis by TEM

1986:97: Millette (editor)

Microbeam Analysis 1986, Proc. 21st Conf. Microbeam Analysis Society

Abstract:

A wide variety of preparation techniques has been employed for analyses of particles and fibers in lung tissues. Wet digestion by sodium or potassium hydroxide, hydrochloric or nitric acid, hydrogen peroxide, enzymes, and sodium hypochlorite solutions have been tried as well as ashing by high-temperature furnace and low-temperature oxygen plasma asher.1 Two of these techniques which have seen recent application and are reportedly free of undesirable effects on inorganic fibers are the sodium hypochlorite digestion (SHD) and the low-temperature oxygen plasma ashing (LTA) technique.2,3 In the present study we have examined the applicability and comparability of these two methods and the problems associated with them, as well as their effects on fiber and ferruginous body concentrations in adjacent human lung tissue sections.

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