Microscope, Vol 56:1, 3-11
To prepare airborne or liquid-borne particles for analysis by transmission electron microscopy (TEM), filtration through filters of polycarbonate (PC) and mixed esters of cellulose (commonly known as mixed cellulose ester – MCE) is often used. Polycarbonate filters are prepared by carbon coating the filters and gently dissolving away the filter material, with an appropriate solvent, leaving the particles caught in a carbon film. Mixed cellulose ester filters are prepared by collapsing the filter, etching the top filter residue, carbon coating the filter and dissolving away the residual filter material. This paper describes the procedures in detail with illustrations of the key steps.
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