“Measurement of Subcellular Ions by X-ray Microanalysis for Evidence of Hepatotoxicity”

1986:98: Millette (editor)

Electron Microscopy in Forensic, Occupational, and Environmental Health Sciences, Plenum Press


The hypothesis that the influx of extracellular Ca2+ across a damaged plasma membrane is the final common pathway for chemically mediated cell death1 has stirred considerable controversy and continued interest in the study of subcellular ion shifts as sensitive indicators of hepatotoxicity caused by environmental pollutants. A number of studies have shown that increases in hepatic mitochondrial calcium result from the ingestion of carbon tetrachloride. 2-4 Quantitative measurement of calcium in subcellular components was accomplished in early studies by using techniques involving whole tissue homogenation and movement of fractionation by centrifugation. In this study x-ray microanalysis of quick-frozen liver tissue, cryo-sectioned for the electron microscope, provided an alternative approach to studying subcellular ion distribution.

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